Coding
E.ssb

Part:BBa_K3790006:Design

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-01)


E.ssb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part.

E.ssb-1      	5-GCAAGCCGTGGTGTTAATAAAGTTATTCTGGTGGGTAATCTGGGTCAAGATCCGGAAG 
E.ssb-2      	5-GGGTCAAGATCCGGAAGTTCGTTATATGCCGAATGGTGGTGCAGTTGCAAATATTACCC
E.ssb-3      	5-GTGCAGTTGCAAATATTACCCTGGCAACCAGCGAAAGCTGGCGTGATAAAGCAACCGG 
E.ssb-4      	5-CGTGATAAAGCAACCGGcGAAATGAAAGAACAGACCGAATGGCATCGTGTTGTTCTG  
E.ssb-5      	5-GGCATCGTGTTGTTCTGTTTGGTAAACTGGCAGAAGTTGCAAGCGAATATCTGCG    
E.ssb-6      	5-GCAAGCGAATATCTGCGTAAAGGTAGCCAGGTTTATATTGAAGGTCAGCTGCGTACCCG
E.ssb-7      	5-ACTTCGGTGGTATAACGATCCTGACCGCTCTGATCGGTCCATTTACGGGTACGCAGCTG
E.ssb-8      	5-CCACCTAACATCTGCATGGTGCCACCAACATTAACAACAACTTCGGTGGTATAACGATC
E.ssb-9      	5-ACCAATATTACCGCCTGCCGGTGCGCCACCACCTTGACGACCACCTAACATCTGCATGG
E.ssb-10     	5-GGCTGCTGAGGCTGACCCCAACCACCTTGCGGCTGACCACCACCAATATTACCGCCTGC
E.ssb-11     	5-TGCGGACGGCTCTGTGCACCACCGCTAAACTGGTTACCGCCTTGAGGCTGCTGAGGCTG
E.ssb-12     	5-CGGTTCATTGCTCGGTGCTGCTGGTGCGCTCTGCTGCGGACGGCTCTGTG         
E.ssb-13     	5-AAACGGGATATCATCATCAAAATCCATAGGCGGTTCATTGCTCGGTGCTGC        

Source

References